Magnetic material based on mesostructured silica functionalized with β-cyclodextrin to extract opium alkaloids in poppy seed infusions

Sample preparation methods tend to be fast, simple, with the use of new adsorbents and lower amounts organic solvents make them more environmentally friendly. In this work, a magnetic solid-phase extraction (MSPE) protocol for quantification six opium alkaloids (OAs) in poppy seeds infusions has been optimised followed by analysis liquid chromatography coupled tandem mass spectrometry (HPLC-MS/MS). For purpose, mesostructured silica composite β-CD (Fe3O4@SiO2@mSiO2@β-CD) was synthesised characterized. To obtain highest level functionalisation, three proportions monomer/material (12.5, 25 100%) were determined, demonstrating that 25% showed level. Adsorption studies carried out water at different pH (1, 2, 7, 9 11) times 5, 10 20 min), confirming high adsorption capacity 1 min. Then, elution conditions MSPE procedure (50 mg material, 2 mL water/EtOH 50% 1% formic acid min). The methodology successful validated low detection limits, negligible matrix effect good recovery values (89–94%). applied home-made four seeds, observing one worrying amount OAs which imply content least legislation.